PT  - JOURNAL ARTICLE
AU  - Morgan Vague
AU  - Gayle Chan
AU  - Cameron Roberts
AU  - Natasja A. Swartz
AU  - Jay L. Mellies
TI  - <em>Pseudomonas</em> isolates degrade and form biofilms on polyethylene terephthalate (PET) plastic
AID  - 10.1101/647321
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 647321
4099  - http://biorxiv.org/content/early/2019/05/24/647321.short
4100  - http://biorxiv.org/content/early/2019/05/24/647321.full
AB  - Bioaugmentation is a possible remediation strategy for the massive amounts of plastic waste in our oceans and landfills. For this study, soil samples were collected from petroleum polluted locations in the Houston, Texas area to isolate microorganisms capable of plastic degradation. Bacteria were propagated and screened for lipase activity, which has been associated with the bacterial degradation of some plastics to date. We identified three lipase-positive Pseudomonas species, and Bacillus cereus as part of two consortia, which we predict enhances biofilm formation and plastic degradation. Lipase-positive consortia bacteria were incubated alongside blank and E.coli controls with UV-irradiated polyethylene terephthalate (PET), high-density polyethylene (HDPE), or low-density polyethylene (LDPE) as sole sources of carbon. Surface degradation of PET plastic was quantified by changes in molecular vibrations by infrared spectroscopy. The bacteria formed biofilms on PET, observed by scanning electron microscopy, and induced molecular changes on the plastic surface, indicating the initial stages of plastic degradation. We also found molecular evidence that one of the Pseudomonas isolates degrades LDPE. To date, lipase positive Pseudomonas spp. degradation of PET has not been well described, and this work highlights the potential for using consortia of common soil bacteria to degrade plastic waste.