RT Journal Article
SR Electronic
T1 Inducible Plasmid Self-Destruction (IPSD) assisted genome engineering in lactobacilli and bifidobacteria
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 649921
DO 10.1101/649921
A1 Zuo, Fanglei
A1 Zeng, Zhu
A1 Hammarström, Lennart
A1 Marcotte, Harold
YR 2019
UL http://biorxiv.org/content/early/2019/05/24/649921.abstract
AB Genome engineering is essential for application of synthetic biology in probiotics including lactobacilli and bifidobacteria. Several homologous recombination system-based mutagenesis tools have been developed for these bacteria but still, have many limitations in different species or strains. Here we developed a genome engineering method based on an inducible self-destruction plasmid delivering homologous DNA into bacteria. Excision of the replicon by induced recombinase facilitates selection of homologous recombination events. This new genome editing tool called Inducible Plasmid Self-Destruction (IPSD) was successfully used to perform gene knock-out and knock-in in lactobacilli and bifidobacteria. Due to its simplicity and universality, the IPSD strategy may provide a general approach for genetic engineering of various bacterial species.