TY - JOUR T1 - The genetic interaction between HIV and the antibody repertoire JF - bioRxiv DO - 10.1101/646968 SP - 646968 AU - Nicolas Strauli AU - Emily Kathleen Fryer AU - Olivia Pham AU - Mohamed Abdel-Mohsen AU - Shelley N. Facente AU - Christopher Pilcher AU - Pleuni Pennings AU - Satish Pillai AU - Ryan D. Hernandez Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/05/24/646968.abstract N2 - The interaction between human immunodeficiency virus (HIV) and the antibody repertoire (AbR) during chronic infection can provide important information for HIV vaccine research, yet has not been well-characterized on a systems level. We deeply sequenced the HIV population and the AbR of ten HIV-infected, antiretroviral (ART)-naïve individuals, each with 10-20 longitudinal samples spanning 4-14 years. Our unbiased sequencing approach identified partitions of AbRs showing evidence of interaction with autologous HIV populations. We show that these HIV-associated partitions are enriched for the V gene segments of known HIV broadly neutralizing antibodies (bnAbs), indicating that the HIV-responding component of the AbR can be identified via time-series genetic data. Despite this evidence for larger-scale AbR/HIV interactions at the sub-population level, we found little to no evidence for coevolution. This suggests that coevolution is either rare, or hard to detect, which has important vaccine design implications. ER -