TY - JOUR T1 - Application of Pharmacogenomics and Bioinformatics to Exemplify the Utility of Human <em>ex vivo</em> Organoculture Models in the Field of Precision Medicine JF - bioRxiv DO - 10.1101/649376 SP - 649376 AU - K Cowan AU - G Macluskie AU - M Finch AU - C.N.A Palmer AU - J Hair AU - M Bylesjo AU - S Lynagh AU - P Brankin AU - M McNeil AU - C Low AU - D Mallinson AU - EM Gourlay AU - H Child AU - L Cheyne AU - DC Bunton Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/05/27/649376.abstract N2 - Here we describe a collaboration between industry, the National Health Service (NHS) and academia that sought to demonstrate how early understanding of both pharmacology and genomics can improve strategies for the development of precision medicines. Diseased tissue ethically acquired from patients suffering from chronic obstructive pulmonary disease (COPD), was used to investigate inter-patient variability in drug efficacy using ex vivo organocultures of fresh lung tissue as the test system. The reduction in inflammatory cytokines in the presence of various test drugs was used as the measure of drug efficacy and the individual patient responses were then matched against genotype and microRNA profiles in an attempt to identify unique predictors of drug responsiveness. Our findings suggest that genetic variation in CYP2E1 and SMAD3 genes may partly explain the observed variation in drug response. ER -