PT  - JOURNAL ARTICLE
AU  - Steffen H. Raun
AU  - Carlos Henriquez Olguín
AU  - Iuliia Karavaeva
AU  - Mona Ali
AU  - Lisbeth L. V. Møller
AU  - Witold Kot
AU  - Josué L. Castro Mejía
AU  - Dennis Sandris Nielsen
AU  - Zach Gerhart-Hines
AU  - Erik A. Richter
AU  - Lykke Sylow
TI  - Housing temperature influences exercise training adaptations in mice
AID  - 10.1101/651588
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 651588
4099  - http://biorxiv.org/content/early/2019/05/27/651588.short
4100  - http://biorxiv.org/content/early/2019/05/27/651588.full
AB  - Exercise training is a powerful means to combat metabolic pathologies. Mice are extensively used to describe the benefits of exercise, but mild cold stress induced by housing temperatures may confound translation to humans. Thermoneutral housing is a strategy to make mice more metabolically similar to humans but its effects on exercise adaptations are unknown. Using voluntary wheel running, we show that thermoneutral housing blunted exercise-induced improvements in insulin action in muscle and adipose tissue. Moreover, thermoneutrality reduced the effects of training on energy expenditure, body composition, muscle and adipose tissue protein expressions, and the gut microbiome. The majority of these thermoneutral-dependent training adaptations could not be ascribed to a lower voluntary running volume. Thus, we conclude that organismal adaptations to exercise training in mice critically depend upon housing temperature. Our findings underscore the importance of housing temperature as an important parameter in the design and interpretation of murine exercise studies.Housing at 30°C blunts several adaptations to exercise training in miceExercise-sensitive protein induction is dampened at 30°C in skeletal muscle30°C-housing blunts training-induced increase in insulin-stimulated glucose uptakeGlucose tolerance is not improved by voluntary exercise training at 30°C housingDecreased running in 30°C housing is not due to overheatingHighlights Graphical abstract