PT  - JOURNAL ARTICLE
AU  - Tsung-Li Liu
AU  - Srigokul Upadhyayula
AU  - Daniel E. Milkie
AU  - Ved Singh
AU  - Kai Wang
AU  - Ian A. Swinburne
AU  - Kishore R. Mosaliganti
AU  - Zach M. Collins
AU  - Tom W. Hiscock
AU  - Jamien Shea
AU  - Abraham Q. Kohrman
AU  - Taylor N. Medwig
AU  - Daphne Dambournet
AU  - Ryan Forster
AU  - Brian Cunniff
AU  - Yuan Ruan
AU  - Hanako Yashiro
AU  - Steffen Scholpp
AU  - Elliot M. Meyerowitz
AU  - Dirk Hockemeyer
AU  - David G. Drubin
AU  - Benjamin L. Martin
AU  - David Q. Matus
AU  - Minoru Koyama
AU  - Sean G. Megason
AU  - Tom Kirchhausen
AU  - Eric Betzig
TI  - Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms
AID  - 10.1101/243352
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 243352
4099  - http://biorxiv.org/content/early/2018/01/09/243352.short
4100  - http://biorxiv.org/content/early/2018/01/09/243352.full
AB  - True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution without inducing undue stress on either. We combined lattice light sheet microscopy with two-channel adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages, and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.One Sentence Summary Combining lattice light sheet microscopy with adaptive optics enables high speed, high resolution in vivo 3D imaging of dynamic processes inside cells under physiological conditions within their parent organisms.