RT Journal Article
SR Electronic
T1 Dense neuronal reconstruction through X-ray holographic nano-tomography
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 653188
DO 10.1101/653188
A1 Pacureanu, Alexandra
A1 Maniates-Selvin, Jasper
A1 Kuan, Aaron T.
A1 Thomas, Logan A.
A1 Chen, Chiao-Lin
A1 Cloetens, Peter
A1 Lee, Wei-Chung Allen
YR 2019
UL http://biorxiv.org/content/early/2019/05/30/653188.abstract
AB Elucidating the structure of neuronal networks provides a foundation for understanding how the nervous system processes information to generate behavior. Despite technological breakthroughs in visible light and electron microscopy, imaging dense nanometer-scale neuronal structures over millimeter-scale tissue volumes remains a challenge. Here, we demonstrate that X-ray holographic nano-tomography is capable of imaging large tissue volumes with sufficient resolution to disentangle dense neuronal circuitry in Drosophila melanogaster and mammalian central and peripheral nervous tissue. Furthermore, we show that automatic segmentation using convolutional neural networks enables rapid extraction of neuronal morphologies from these volumetric datasets. The technique we present allows rapid data collection and analysis of multiple specimens, and can be used correlatively with light microscopy and electron microscopy on the same samples. Thus, X-ray holographic nano-tomography provides a new avenue for discoveries in neuroscience and life sciences in general.