PT  - JOURNAL ARTICLE
AU  - Sarah A. Overall
AU  - Jugmohit S. Toor
AU  - Stephanie Hao
AU  - Mark Yarmarkovich
AU  - Son Nguyen
AU  - Alberto S. Japp
AU  - Danai Moschidi
AU  - Michael R. Betts
AU  - John M. Maris
AU  - Peter Smibert
AU  - Nikolaos G. Sgourakis
TI  - High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
AID  - 10.1101/653477
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 653477
4099  - http://biorxiv.org/content/early/2019/05/30/653477.short
4100  - http://biorxiv.org/content/early/2019/05/30/653477.full
AB  - Peptide exchange technologies are essential for the generation of pMHC-multimer libraries, used to probe highly diverse, polyclonal TCR repertoires. Using the molecular chaperone TAPBPR, we present a robust method for the capture of stable, empty MHC-I molecules which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Combined with tetramer barcoding using multi-modal cellular indexing technology (ECCITE-seq), our approach allows a combined analysis of TCR repertoires and other T-cell transcription profiles together with their cognate pMHC-I specificities in a single experiment.