RT Journal Article
SR Electronic
T1 Cristae undergo continuous cycles of fusion and fission in a MICOS-dependent manner
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 654541
DO 10.1101/654541
A1 Arun Kumar Kondadi
A1 Ruchika Anand
A1 Sebastian Hänsch
A1 Jennifer Urbach
A1 Thomas Zobel
A1 Dane M. Wolf
A1 Mayuko Segawa
A1 Marc Liesa
A1 Orian S. Shirihai
A1 Stefanie Weidtkamp-Peters
A1 Andreas S. Reichert
YR 2019
UL http://biorxiv.org/content/early/2019/05/30/654541.abstract
AB The mitochondrial inner membrane can reshape under different physiological conditions. How and at which frequency this occurs in vivo and what are the molecular players involved is unknown. Here we show using state-of-the-art live-cell stimulated emission depletion (STED) super-resolution nanoscopy that crista junctions (CJs) are dynamically fusing and dividing in a reversible and balanced manner at a timescale of seconds. CJ dynamics is strongly reduced in the absence of the MICOS subunit MIC13. Staining of the cristae membrane using different protein markers or two inner mitochondrial membrane-specific dyes revealed that cristae also undergo continuous cycles of fusion and fission. These processes are dependent on MIC13 and occur at a timescale of seconds, resembling CJ dynamics. Our data further suggest that MIC60 acts as a docking platform pioneering CJ formation. Overall, by employing a variety of advanced imaging techniques including FRAP (Fluorescence-Recovery-After Photobleaching), SPT (Single-Particle-Tracking), live-cell STED and confocal Airyscan microscopy we demonstrate that cristae undergo continuous cycles of fusion and fission in a manner that is mechanistically linked to CJ formation and dynamics.