@article {Challis246405, author = {Rosemary C Challis and Sripriya Ravindra Kumar and Ken Y Chan and Collin Challis and Min J Jang and Pradeep S Rajendran and John D Tompkins and Kalyanam Shivkumar and Benjamin E Deverman and Viviana Gradinaru}, title = {Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration}, elocation-id = {246405}, year = {2018}, doi = {10.1101/246405}, publisher = {Cold Spring Harbor Laboratory}, abstract = {We recently developed novel AAV capsids for efficient and noninvasive gene transfer across the central and peripheral nervous systems. In this protocol, we describe how to produce and systemically administer AAV-PHP viruses to label and/or genetically manipulate cells in the mouse nervous system and organs including the heart. The procedure comprises three separate stages: AAV production, intravenous delivery, and evaluation of transgene expression. The protocol spans eight days, excluding the time required to assess gene expression, and can be readily adopted by laboratories with standard molecular and cell culture capabilities. We provide guidelines for experimental design and choosing the capsid, cargo, and viral dose appropriate for the experimental aims. The procedures outlined here are adaptable to diverse biomedical applications, from anatomical and functional mapping to gene expression, silencing, and editing.}, URL = {https://www.biorxiv.org/content/early/2018/01/11/246405}, eprint = {https://www.biorxiv.org/content/early/2018/01/11/246405.full.pdf}, journal = {bioRxiv} }