RT Journal Article
SR Electronic
T1 The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 658492
DO 10.1101/658492
A1 Xiangduo Kong
A1 Yen-Yun Chen
A1 Jianhuang Lin
A1 Ebony Flowers
A1 Eric Van Nostrand
A1 Steven M. Blue
A1 Jonathan Chau
A1 Christopher I-Hsing Ma
A1 Isaiah Mohr
A1 Ryan Thai
A1 Chengguo Yao
A1 Alexander R. Ball, Jr.
A1 Richard Chien
A1 Shimako Kawauchi
A1 Rosalyn Santos
A1 Anne L. Calof
A1 Arthur D. Lander
A1 Yongsheng Shi
A1 Mitsuru Okuwaki
A1 Gene W. Yeo
A1 Kyoko Yokomori
YR 2019
UL http://biorxiv.org/content/early/2019/06/03/658492.abstract
AB NIPBL is an essential loader of cohesin to mediate sister chromatid cohesion and chromatin loop organization. NIPBL mutations cause Cornelia de Lange Syndrome. How NIPBL’s genomic localization is specified is not fully understood. We found that NIPBL localizes to the nucleolus in an RNA-dependent manner and binds directly to ribosomal RNA (rRNA). We identified two RNA binding domains in NIPBL in vitro, both of which are required for efficient rRNA binding in vivo. NIPBL binds to ribosomal DNA (rDNA) in an RNA-stimulated manner, recruits PAF1 and promotes pre-rRNA transcription. Stress that inhibits rRNA synthesis displaces NIPBL from the nucleolus and rDNA. Interestingly, treacle, mutated in Treacher Collins syndrome, tightly binds to and recruits NIPBL to the nucleolus, nucleolar organizer regions, and the stress-induced nucleolar cap. The results reveal that a subpopulation of NIPBL is recruited to the nucleolus through its interaction with RNA and treacle and regulates pre-rRNA transcription.