RT Journal Article
SR Electronic
T1 A DNA-based synthetic apoptosome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 660183
DO 10.1101/660183
A1 Bas J.H.M. Rosier
A1 Albert J. Markvoort
A1 Berta Gumí-Audenis
A1 Job A.L. Roodhuizen
A1 Anniek den Hamer
A1 Luc Brunsveld
A1 Tom F.A. de Greef
YR 2019
UL http://biorxiv.org/content/early/2019/06/04/660183.abstract
AB Living cells are able to regulate key cellular processes by physically assembling signaling components on dedicated molecular platforms. The spatial organization of proteins in these higher-order signaling complexes facilitates proximity-driven activation and inhibition events, allowing tight regulation of the flow of information. Here, we employ the programmability and modularity of DNA origami as a controllable molecular platform for studying protein-protein interactions involved in intracellular signaling. Specifically, we engineer a synthetic, DNA origami-based version of the apoptosome, a large multi-protein signaling complex that regulates apoptosis by co-localization of multiple caspase-9 monomers. Our in vitro characterization using both wildtype caspase-9 monomers and inactive mutants tethered to a DNA origami platform directly demonstrates that enzymatic activity is induced by proximity-driven dimerization with asymmetric, half-of-sites reactivity. Additionally, experimental results supported by a detailed thermodynamic model reveal a multivalent activity enhancement in tethered caspase-9 oligomers of three and four enzymes, partly originating from a statistical increase in the number of active catalytic units in higher-order enzyme clusters. Our results offer fundamental insights in caspase-9 activity regulation and demonstrate that DNA origami provides a modular platform to construct and characterize higher-order signaling complexes. The engineered DNA-based protein assembly platform has the potential to be broadly applied to inform the function of other important multi-enzyme assemblies involved in inflammation, innate immunity, and necrosis.