RT Journal Article
SR Electronic
T1 Resistance gene discovery and cloning by sequence capture and association genetics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 248146
DO 10.1101/248146
A1 Sanu Arora
A1 Burkhard Steuernagel
A1 Sutha Chandramohan
A1 Yunming Long
A1 Oadi Matny
A1 Ryan Johnson
A1 Jacob Enk
A1 Sambasivam Periyannan
A1 M. Asyraf Md Hatta
A1 Naveenkumar Athiyannan
A1 Jitender Cheema
A1 Guotai Yu
A1 Ngonidzashe Kangara
A1 Sreya Ghosh
A1 Les J. Szabo
A1 Jesse Poland
A1 Harbans Bariana
A1 Jonathan D. G. Jones
A1 Alison R. Bentley
A1 Mick Ayliffe
A1 Eric Olson
A1 Steven S. Xu
A1 Brian J. Steffenson
A1 Evans Lagudah
A1 Brande B. H. Wulff
YR 2018
UL http://biorxiv.org/content/early/2018/01/15/248146.abstract
AB Genetic resistance is the most economic and environmentally sustainable approach for crop disease protection. Disease resistance (R) genes from wild relatives are a valuable resource for breeding resistant crops. However, introgression of R genes into crops is a lengthy process often associated with co-integration of deleterious linked genes1, 2 and pathogens can rapidly evolve to overcome R genes when deployed singly3. Introducing multiple cloned R genes into crops as a stack would avoid linkage drag and delay emergence of resistance-breaking pathogen races4. However, current R gene cloning methods require segregating or mutant progenies5–10, which are difficult to generate for many wild relatives due to poor agronomic traits. We exploited natural pan-genome variation in a wild diploid wheat by combining association genetics with R gene enrichment sequencing (AgRenSeq) to clone four stem rust resistance genes in <6 months. RenSeq combined with diversity panels is therefore a major advance in isolating R genes for engineering broad-spectrum resistance in crops.