PT  - JOURNAL ARTICLE
AU  - M. Boone
AU  - P. Ramasamy
AU  - D. Maddelein
AU  - D. Turan
AU  - E. Vandermarliere
AU  - W. Vranken
AU  - N. Callewaert
TI  - Beyond the ribosome: proteome-wide secretability studies with SECRiFY
AID  - 10.1101/241349
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 241349
4099  - http://biorxiv.org/content/early/2018/01/15/241349.1.short
4100  - http://biorxiv.org/content/early/2018/01/15/241349.1.full
AB  - While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis up to the ribosome-associated stages are now widely available, we still lack global approaches to assay post-ribosomal processes, in particular those occurring in the eukaryotic secretory system. We developed SECRiFY to simultaneously assess the secretability of >105 heterologous protein fragments by two yeast species, S. cerevisiae and P. pastoris, using custom fragment libraries, surface display and a sequencing-based readout. SECRiFY generates datasets that enable datamining into protein features underlying secretability, and it will enable studies of the impact of secretory system perturbation on the secretable proteome.