TY - JOUR T1 - Endogenous pioneer neutrophils release NETs during the swarming response in zebrafish JF - bioRxiv DO - 10.1101/521450 SP - 521450 AU - Hannah M. Isles AU - Catherine A. Loynes AU - NoƩmie Hamilton AU - Clare F. Muir AU - Anastasia Kadochnikova AU - Katherine M. Henry AU - Visakan Kadirkamanathan AU - Stephen A. Renshaw AU - Philip M. Elks Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/06/05/521450.abstract N2 - Neutrophils are rapidly recruited to inflammatory sites where they coordinate their migration to form clusters, a process termed neutrophil swarming. The factors which modulate neutrophil swarming during its early stages are not fully understood, requiring the development of new in vivo models. Using transgenic zebrafish larvae to study endogenous neutrophil migration in a tissue damage model, we demonstrate that neutrophil swarming is a conserved process in zebrafish immunity, sharing essential features with mammalian systems. We show that neutrophil swarms initially develop around a pioneer neutrophil, in a three-phase sequence of events. By adopting a high-resolution confocal microscopy approach, we observed the release of cell fragments by early swarming neutrophils. We developed a neutrophil specific histone H2A transgenic reporter line TgBAC(mpx:GFP)i114;Tg(lyz:H2A-mCherry)sh530 to study neutrophil extracellular traps (NETs), and found that endogenous neutrophils recruited to sites of tissue damage released NETs at the start of the swarming process. The optical transparency achieved using the zebrafish model has provided some of the highest resolution imaging of NET release in vivo to date. Using a combination of transgenic reporter lines and DNA intercalating agents, we demonstrate that pioneer neutrophils release extracellular traps during the swarming response, suggesting that cell death signalling via NETosis might be important in driving the swarming response. ER -