RT Journal Article
SR Electronic
T1 Luminal MCF-12A and myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 248765
DO 10.1101/248765
A1 Anne Weber-Ouellette
A1 Mélanie Busby
A1 Isabelle Plante
YR 2018
UL http://biorxiv.org/content/early/2018/01/16/248765.abstract
AB The mammary gland is a complex organ, structured in a ramified epithelium supported by the stroma. The epithelium’s functional unit is the bilayered acinus, made of a layer of luminal cells surrounded by a layer of basal cells mainly composed of myoepithelial cells. The aim of this study was to develop a reproducible and manipulable three-dimensional co-culture model of the bilayered acinus in vitro to study the interactions between the two layers. Two different combinations of cell lines were co-cultured in Matrigel: SCp2 and SCg6 mice cells, or MCF-12A and Hs 578Bst human cell lines. Cell ratios and Matrigel concentration were optimized. The resulting acini were analysed by confocal microscopy using epithelial (E-cadherin) and myoepithelial (α-smooth muscle actin) markers. SCp2 and SCg6 cells formed distinct three-dimensional structures, whereas MCF-12A and Hs 578Bst cells formed some bilayered acini. This in vitro bilayered acini model will allow us to understand the role of interactions between luminal and myoepithelial cells in the normal breast development.