RT Journal Article SR Electronic T1 Development of a multi-locus typing scheme for an Enterobacteriaceae linear plasmid that mediates inter-species transfer of flagella JF bioRxiv FD Cold Spring Harbor Laboratory SP 664508 DO 10.1101/664508 A1 James Robertson A1 Janet Lin A1 Amie Wren-Hedegus A1 Gitanjali Arya A1 Catherine Carrillo A1 John H.E. Nash YR 2019 UL http://biorxiv.org/content/early/2019/06/07/664508.abstract AB Due to the public health importance of flagellar genes for typing, it is important to understand mechanisms that could alter their expression or presence. Phenotypic novelty in flagellar genes arise predominately through accumulation of mutations but horizontal transfer is known to occur. A linear plasmid termed pBSSB1 previously identified in Salmonella Typhi, was found to encode a flagellar operon that can mediate phase variation, which results in the rare z66 flagella phenotype. The identification and tracking of homologs of pBSSB1 is limited because it falls outside the normal replicon typing schemes for plasmids. Here we report the generation of nine new pBSSB1-family sequences using Illumina and Nanopore sequence data. Homologs of pBSSB1 were identified in 154 genomes representing 25 distinct serotypes from 67,758 Salmonella public genomes. Pangenome analysis of pBSSB1-family contigs was performed using Roary and we identified three core genes amenable to a minimal MLST scheme. Population structure analysis based on the newly developed MLST scheme identified three major lineages representing 35 sequence types, and the distribution of these sequence types was found to span multiple serovars across the globe. This MLST scheme has shown utility in tracking and subtyping pBSSB1-family plasmids and it has been incorporated into the plasmid MLST database under the name “pBSSB1-family”.cgMLSTcore gene multi-locus sequence typingMLSTmulti-locus sequence typingSTsequence typeWGSwhole genome sequencingWKLWhite-Kauffman Le Minor serotyping scheme