RT Journal Article
SR Electronic
T1 Engineered Retroviruses as Fluorescent Biological Reference Particles for Small Particle Flow Cytometry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 614461
DO 10.1101/614461
A1 Vera A. Tang
A1 Anna K. Fritzsche
A1 Tyler M. Renner
A1 Dylan Burger
A1 Edwin van der Pol
A1 Joanne A. Lannigan
A1 George C. Brittain
A1 Joshua A. Welsh
A1 Jennifer C. Jones
A1 Marc-André Langlois
YR 2019
UL http://biorxiv.org/content/early/2019/06/07/614461.abstract
AB There has been renewed interest in the use of flow cytometry for single particle phenotypic analysis of particles in the nanometer size-range such as viruses, organelles, bacteria and extracellular vesicles (EVs). However, many of these particles are smaller than 200 nm in diameter, which places them at the limit of detection for many commercial flow cytometers. The use of reference particles of diameter, fluorescence, and light-scattering properties akin to those of the small biological particles being studied is therefore imperative for accurate and reproducible data acquisition and reporting across different instruments and analytical technologies. We show here that an engineered murine leukemia virus (MLV) can act as a fluorescence reference particle for other small particles such as retroviruses and EVs. More specifically, we show that engineered MLV is a highly monodisperse enveloped particle that can act as a surrogate to demonstrate the various effects of antibody labeling on the physical properties of small biological particles in a similar diameter range.