@article {Garretson249615, author = {Tyler A. Garretson and Joshua G. Petrie and Emily T. Martin and Arnold S. Monto and Scott E. Hensley}, title = {Identification of human vaccinees that possess antibodies targeting the egg-adapted hemagglutinin receptor binding site of an H1N1 influenza vaccine strain}, elocation-id = {249615}, year = {2018}, doi = {10.1101/249615}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Human influenza viruses passaged in eggs often acquire mutations in the hemagglutinin (HA) receptor binding site (RBS). To determine if egg-adapted H1N1 vaccines commonly elicit antibodies targeting the egg-adapted RBS of HA, we completed hemagglutinin-inhibition assays with A/California/7/2009 HA and egg-adapted A/California/7/2009-X-179A HA using sera collected from 159 humans vaccinated with seasonal influenza vaccines during the 2015-16 season. We found that ~5\% of participants had >=4-fold higher antibody titers to the egg-adapted viral strain compared to wild type viral strain. We used reverse-genetics to demonstrate that a single egg-adapted HA RBS mutation (Q226R) was responsible for this phenotype.}, URL = {https://www.biorxiv.org/content/early/2018/01/17/249615}, eprint = {https://www.biorxiv.org/content/early/2018/01/17/249615.full.pdf}, journal = {bioRxiv} }