RT Journal Article SR Electronic T1 Single molecule sequencing of THCA synthase reveals copy number variation in modern drug-type Cannabis sativa L JF bioRxiv FD Cold Spring Harbor Laboratory SP 028654 DO 10.1101/028654 A1 McKernan, Kevin J. A1 Helbert, Yvonne A1 Tadigotla, Vasisht A1 McLaughlin, Stephen A1 Spangler, Jessica A1 Zhang, Lei A1 Smith, Douglas YR 2015 UL http://biorxiv.org/content/early/2015/10/08/028654.abstract AB Cannabinoid expression is an important genetically determined feature of cannabis that presents clinical and legal implications for patients seeking cannabinoid specific therapies like Cannabidiol (CBD).Cannabinoid, terpenoid, and flavonoid marker assisted selection can accelerate breeding efforts by offering genetic tools to select for desired traits at an early stage in growth. To this end, multiple models for chemotype inheritance have been described suggesting a complex picture for chemical phenotype determination.Here we explore the potential role of copy number variation of THCA Synthase using phased single molecule sequencing and demonstrate that copy number and sequence variation of this gene is common and suggests a more nuanced view of chemotype prediction.