TY - JOUR T1 - PRC1-dependent compaction of Hox gene clusters prevents transcriptional derepression during early <em>Drosophila</em> embryogenesis JF - bioRxiv DO - 10.1101/250183 SP - 250183 AU - Thierry Cheutin AU - Giacomo Cavalli Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/01/18/250183.abstract N2 - Polycomb-group (PcG) proteins are conserved chromatin factors that maintain the silencing of key developmental genes, notably the Hox gene clusters, outside of their expression domains [1-3]. Polycomb repressive complex 2 (PRC2) trimethylates lysine K27 of histone H3 [4], and PRC1 collaborates with PRC2 in gene silencing. Genome-wide studies have revealed large H3K27me3 chromatin domains bound by PcG proteins, and Polycomb domains fold into distinct nuclear structures [5-9]. Although PRC1 is involved in chromatin compaction [10-16], it is unknown whether PRC1-dependent transcriptional silencing is a consequence of its role on higher-order chromatin folding. This is because depletion of PRC1 proteins typically induces both chromatin unfolding and ectopic transcription, and ectopic transcription can open chromatin by itself. To disentangle these two components, we analysed the temporal effects of two PRC1 proteins, Polyhomeotic (Ph) and Polycomb (Pc), on Hox gene clusters during Drosophila embryogenesis. We show that the absence of Ph or Pc affects the higher-order chromatin folding of Hox clusters prior to ectopic Hox gene transcription, demonstrating that PRC1 primary function during early embryogenesis is to compact its target chromatin. During later embryogenesis, we observed further chromatin opening at Hox complexes in both Ph and Pc mutants, which was coupled to strong deregulation of Hox genes at this stage of development. Moreover, the differential effects of Ph and Pc on Hox cluster folding matches the differences in ectopic Hox gene expression observed in these two mutants, suggesting that the degree of Hox derepression in PcG mutants depends on the degree of structural constraints imposed by each PcG component. In summary, our data demonstrate that binding of PRC1 to large genomic domains during early embryogenesis induces the formation of compact chromatin to prevent ectopic gene expression at later time-points. Thus, epigenetic mechanisms such as Polycomb mediated silencing act by folding chromatin domains and impose an architectural layer to gene regulation. ER -