RT Journal Article
SR Electronic
T1 Assessment of two immunoassays for detection of IgM antibodies to scrub typhus using a serum panel
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 667410
DO 10.1101/667410
A1 Divyaa Elangovan
A1 Susmitha Perumalla
A1 Winsley Rose
A1 Valsan Philip Verghese
A1 M.S. Gowri
A1 John Antony Jude Prakash
YR 2019
UL http://biorxiv.org/content/early/2019/06/11/667410.abstract
AB Introduction Scrub typhus is a vector borne zoonotic disease caused by Orientia tsutsugamushi, endemic to tsutsugamushi triangle. As the characteristic eschar is not always present, laboratory testing especially serological assay are the main stay of diagnosis.Materials and methods A total of 346 well-characterized sera from normals and patients with scrub typhus, malaria, dengue, enteric fever and gram negative septicaemia were tested for IgM antibodies by ST IgM ELISA and ST Ig M ICTResults The sensitivity and specificity of Scrub typhus IgM ICT and ELISA were 98.7, 96.3 and 97.4, 99.3 respectively. The IgM ICT and ELISA had a excellent concordance (99%) and a very high negative predictive value.Conclusion The findings from this study suggest that IgM ICT and IgM ELISA can be used interchangeably for serodiagnosis of scrub typhus in resource poor settings.