PT  - JOURNAL ARTICLE
AU  - Daniel J. Merk
AU  - Pengcheng Zhou
AU  - Samuel M. Cohen
AU  - Maria F. Pazyra-Murphy
AU  - Grace H. Hwang
AU  - Kristina J. Rehm
AU  - Jose Alfaro
AU  - Xuesong Zhao
AU  - Eunyoung Park
AU  - Pin-Xian Xu
AU  - Jennifer A. Chan
AU  - Michael J. Eck
AU  - Kellie J. Nazemi
AU  - Rosalind A. Segal
TI  - The Eya1 phosphatase mediates Shh-driven symmetric cell division of cerebellar granule cell precursors
AID  - 10.1101/668277
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 668277
4099  - http://biorxiv.org/content/early/2019/06/11/668277.short
4100  - http://biorxiv.org/content/early/2019/06/11/668277.full
AB  - During neural development, stem and precursor cells can divide either symmetrically or asymmetrically. The transition between symmetric and asymmetric cell divisions is a major determinant of precursor cell expansion and neural differentiation, but the underlying mechanisms that regulate this transition are not well understood. Here, we identify the Sonic hedgehog (Shh) pathway as a critical determinant regulating the mode of division of cerebellar granule cell precursors (GCPs). Using partial gain and loss of function mutations within the Shh pathway, we show that pathway activation determines spindle orientation of GCPs, and that mitotic spindle orientation directly correlates with the mode of division. Mechanistically, we show that the phosphatase Eya1 is essential for implementing Shh-dependent GCP spindle orientation. We identify atypical protein kinase C (aPKC) as a direct target of Eya1 activity and show that Eya1 dephosphorylates Threonine (T410) in the activation loop of this polarity complex component. Thus, Eya1 inactivates the cell polarity complex, resulting in reduced phosphorylation of Numb and other components that regulate the mode of division. This Eya1-dependent cascade is critical in linking spindle orientation, cell cycle exit and terminal differentiation. Together these findings demonstrate that a Shh-Eya1 regulatory axis selectively promotes symmetric cell divisions during cerebellar development by coordinating spindle orientation and cell fate determinants.Summary statement Biological responses to Shh signaling are specified by the magnitude of pathway activation and the cellular context. This study shows that potent Shh signaling regulates mitotic orientation and symmetric division of cerebellar granule cell precursors in a process that requires the phosphatase Eya1 and unequal distribution of cell fate determinants to daughter cells.