RT Journal Article SR Electronic T1 Irreversible temperature gating in trpv1 sheds light on channel activation JF bioRxiv FD Cold Spring Harbor Laboratory SP 251124 DO 10.1101/251124 A1 Ana Sánchez-Moreno A1 Eduardo Guevara-Hernández A1 Ricardo Contreras-Cervera A1 Gisela Rangel-Yescas A1 Ernesto Ladrón-de-Guevara A1 Tamara Rosenbaum A1 León D. Islas YR 2018 UL http://biorxiv.org/content/early/2018/01/20/251124.abstract AB Temperature activated TRP channels or thermoTRPs are among the only proteins that can directly convert temperature changes into changes in channel open probability. In spite of a wealth of information, including several experimentally determined structural models of TRP channels, the mechanism of temperature activation remains unknown. We have carefully characterized the repeated activation of TRPV1 by thermal stimuli and discovered a previously unknown inactivation process, which is irreversible. This inactivation is associated with specific conformational changes in the membrane proximal domain. We propose that this form of gating in TRPV1 channels is a consequence of the heat absorption process that leads to channel opening.