RT Journal Article
SR Electronic
T1 Large differences in small RNA composition between human biofluids
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 251496
DO 10.1101/251496
A1 Paula M. Godoy
A1 Nirav R. Bhakta
A1 Andrea J. Barczak
A1 Hakan Cakmak
A1 Susan Fisher
A1 Tippi C. Mackenzie
A1 Tushar Patel
A1 Richard W. Price
A1 James F. Smith
A1 Prescott G. Woodruff
A1 David J. Erle
YR 2018
UL http://biorxiv.org/content/early/2018/01/22/251496.1.abstract
AB Extracellular miRNAs and other small RNAs are implicated in cellular communication and may be useful as disease biomarkers. We systematically compared small RNAs in 12 human biofluid types using RNA-seq. miRNAs and tRNA-derived RNAs (tDRs) accounted for the majority of mapped reads in all biofluids, but the ratio of miRNA to tDR reads varied from 72 in plasma to 0.004 in bile. miRNA levels were highly correlated across all biofluids but levels of some miRNAs differed markedly between biofluids. tDR populations differed extensively between biofluids. Y RNA fragments were seen in all biofluids and accounted for >10% of reads in blood plasma, serum, and CSF. Reads mapping exclusively to piRNAs were very rare except in seminal plasma. These results demonstrate extensive differences in small RNAs between human biofluids and provide a useful resource for investigating extracellular RNA biology and developing biomarkers.