RT Journal Article
SR Electronic
T1 Small-molecule control of super-Mendelian inheritance in gene drives
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 665620
DO 10.1101/665620
A1 López Del Amo, Víctor
A1 Leger, Brittany S.
A1 Cox, Kurt J.
A1 Gill, Shubhroz
A1 Bishop, Alena L.
A1 Scanlon, Garrett D.
A1 Walker, James A.
A1 Gantz, Valentino M.
A1 Choudhary, Amit
YR 2019
UL http://biorxiv.org/content/early/2019/06/14/665620.abstract
AB By surpassing the 50% inheritance limit of Mendel’s law of independent assortment, CRISPR-based gene drives have the potential to fight vector-borne diseases or suppress crop pests. However, contemporary gene drives could spread unchecked, posing safety concerns that limit their use in both laboratory and field settings. Current technologies also lack chemical control strategies, which could be applied in the field for dose, spatial and temporal control of gene drives. We describe in Drosophila the first gene-drive system controlled by an engineered Cas9 and a synthetic, orally-available small molecule. Graphical Abstract