RT Journal Article
SR Electronic
T1 Comparison of Small Gut and Whole Gut Microbiota of First-Degree Relatives with Adult Celiac Disease Patients and Controls
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 227272
DO 10.1101/227272
A1 Sudarshan A. Shetty
A1 Rahul Bodkhe
A1 Dhiraj P. Dhotre
A1 Anil K. Verma
A1 Khushbo Bhatia
A1 Asha Mishra
A1 Gurvinder Kaur
A1 Pranav Pande
A1 Dhinoth K. Bangarusamy
A1 Beena P. Santosh
A1 Rajadurai C. Perumal
A1 Vineet Ahuja
A1 Yogesh S. Shouche
A1 Govind K. Makharia
YR 2018
UL http://biorxiv.org/content/early/2018/01/23/227272.abstract
AB Objectives Gut microbiota gets altered in patients with celiac disease (CeD) and whether these microbiota changes are the cause or effect of the disease is not well understood to date. The first degree relatives (FDRs) of CeD patients are genetically susceptible and may represent a pre-diseased state. Therefore, understanding differences in duodenal and faecal microbiota composition between the FDR and CeD subjects is of interest. To investigate this, we characterised the microbiota in duodenal biopsies and faeces of CeD patients (n = 23), FDRs (n = 15) and control subjects (DC, n= 24) by 16S rRNA gene sequencing.Results Duodenal biopsies showed more diverse pattern in microbial community composition and structure than faecal samples. In duodenal biopsies, 52 OTUs and 41 OTUs were differentially abundant between the FDR and DC group, and between the FDR and CeD group respectively (p < 0.01). In faecal samples, 30 OTUs were differentially abundant between FDR and DC, and 81 between FDR and CeD (p < 0.01). Predicted metagenomes from duodenal microbiomes of FDR and CeD showed a lower genetic potential for metabolizing gluten as compared to controls.Conclusions The microbial communities of FDR and CeD groups are more similar to each other than to the control groups. Significant differences at OTU level suggest that specific bacterial taxa may be important for pathogenesis of CeD. Moreover, the predicted differences in gluten metabolism potential by the FDR and CeD microbiota point towards the need for investigating functional capabilities of specific bacterial taxa in healthy FDR and CeD patients.CeDCeliac diseaseDCDiseased controls (dyspeptic)FDRFirst degree relativesOTUOperational taxonomic unitPERMANOVAPermutational multivariate analysis of variancerRNARibosomal Ribonucleic acidPCoAPrincipal coordinates analysisCCACanonical correspondence analysis