PT  - JOURNAL ARTICLE
AU  - Ziying Hu
AU  - Daqi Wang
AU  - Chengdong Zhang
AU  - Shuai Wang
AU  - Siqi Gao
AU  - Linghui Hou
AU  - Hongyan Wang
AU  - Yongming Wang
TI  - Diverse noncanonical PAMs recognized by SpCas9 in human cells
AID  - 10.1101/671503
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 671503
4099  - http://biorxiv.org/content/early/2019/06/14/671503.short
4100  - http://biorxiv.org/content/early/2019/06/14/671503.full
AB  - The CRISPR/Cas9 system derived from Streptococcus pyogenes (SpCas9) provides unprecedented genome editing capabilities, but the potential for off-target mutations limits its application. In addition to NGG protospacer adjacent motif (PAM), off-target mutations are also associated with noncanonical PAMs, which have not yet been systematically evaluated. Here, we developed a highly sensitive approach that allows systematically analyzing PAM sequences in human cells, and identified multiple alternative PAMs recognized by SpCas9.