TY - JOUR T1 - SlARF10, an auxin response factor, is required for chlorophyll and sugar accumulation during tomato fruit development JF - bioRxiv DO - 10.1101/253237 SP - 253237 AU - Lihua Mei AU - Yujin Yuan AU - Mengbo Wu AU - Zehao Gong AU - Qian Zhang AU - Fengqing Yang AU - Qiang Zhang AU - Yingqing Luo AU - Xin Xu AU - Wenfa Zhang AU - Mingjun Miao AU - Zhengguo Li AU - Wei Deng Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/01/24/253237.abstract N2 - Tomato green fruits photosynthesis contributes to fruit growth and carbon economy. Tomato auxin response factor 10 (SlARF10) is one of the members of ARF family. Our results showed that SlARF10 locates in the nucleus and has no transcriptional activity. SlARF10 was expressed in various tomato tissues, but highly expressed in green fruit. Up-regulation of SlARF10 produced dark green phenotype of fruits, whereas down-regulation of SlARF10 had light green phenotype. Autofluorescence and chlorophyll content analysis confirmed the phenotypes, which indicated that SlARF10 plays an important role in chlorophyll accumulation in tomato fruits. Up-regulation of SlARF10 increased the photochemical potential in tomato leaves and fruits. Furthermore, the SlARF10 up-regulating lines displayed improved accumulation of starch in fruits, whereas SlARF10 suppressed lines had inhibited starch accumulation. Up-regulation of SlARF10 increased the expression of AGPases, the starch biosynthesis genes. SlARF10 up-regulating lines had increased accumulation of SlGLK1 and SlGLK2 transcripts in fruits. The promoter sequence of SlGLK1 gene had two conserved ARF binding sites. SlARF10 may regulate the expression of SlGLK1, thus controlling chlorophyll accumulation, photosynthesis rates and sugars synthesis in fruits. Our study provided more insight on the link between auxin signaling, chloroplastic activity and sugar metabolism during the development of tomato fruits.ARFsAuxin Response FactorsRNAiRNA interferenceGLKGOLDEN2-LIKEDET1/hp2The DE-ETIOLATED 1DDB1UV-DAMAGED DNA-BINDING PROTEIN 1KNOXsClass I KNOTTED1-LIKE HOMEOBOXGC-MSGas Chromatography-Mass SpectrometryqRT-PCRQuantitative real time PCRTFsTranscription factorsWTWild-typeMRMiddle regionDB domainDNA binding domainCTDC-terminal interaction domainADTranscriptional activatorsRDTranscriptional repressorsB3N-terminal DNA-binding domainHighlight SlARF10 played an important role in the chlorophyll accumulation and photosynthesis in tomato fruits. SlARF10 was involved in starch accumulation by controlling the expression of starch synthesis related enzyme genes. SlARF10 may regulate the expression of SlGLK1, thus controlling chlorophyll accumulation, photosynthesis rates and sugars synthesis in tomato fruits. ER -