PT  - JOURNAL ARTICLE
AU  - Harsh Pandey
AU  - Shivani Srivastava
AU  - Surabhi Singh
AU  - Yamini BhusanTripathi
TI  - Histopathological study of different organs of charles foster strain rat under the exposure of <em>Pueraria tuberosa</em>
AID  - 10.1101/671529
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 671529
4099  - http://biorxiv.org/content/early/2019/06/14/671529.short
4100  - http://biorxiv.org/content/early/2019/06/14/671529.full
AB  - Objective The present study was undertaken to investigate the safe doses of Pueraria tuberosa water extract (PTWE) on different organs.Methodology Haematoxylin and eosin staining was used to study the morphological alterations in heart, intestine, testis, adrenal gland and spleen. Followed the OECD guidelines 407 of repeated toxicity with respect to the selection of dose and days for different organs. The selected doses of PTWE were 250, 500, 1000 and 2000 mg/kg bw and the durations selected were 7, 14, 21 and 28 days.Result According to the obtained results, we have found no any types of adverse alteration in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, semeniferous tubules and spermatozoa count were normal in testis, all three zones of adrenal gland were normally identified and no any adverse sign of pulps in spleen was seen in all treated groups of PTWEConclusion There was no any types of adverse morphological alteration found in any organs. The drug PTWE are safe at 1000 mg/kg bw upto 28 days and 2000 mg/ kg bw upto 21 days respectively.