RT Journal Article
SR Electronic
T1 In-vivo targeted tagging of RNA isolates cell specific transcriptional responses to environmental stimuli and identifies liver-to-adipose RNA transfer
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 670398
DO 10.1101/670398
A1 J. Darr
A1 M. Lassi
A1 Archana Tomar
A1 R. Gerlini
A1 F. Scheid
A1 M. Hrabě de Angelis
A1 M. Witting
A1 R. Teperino
YR 2019
UL http://biorxiv.org/content/early/2019/06/14/670398.abstract
AB Bio-fluids contain various circulating cell-free RNA transcripts (ccfRNAs). The composition of these ccfRNAs varies between bio-fluids and constitute tantalizing biomarker candidates for several pathologies. ccfRNAs have also been demonstrated as mediators of cellular communication, yet little is known about their function in physiological and developmental settings and most works are limited to in-vitro studies. Here, we have developed iTAG-RNA, a novel method for the unbiased tagging of RNA transcripts in mice in-vivo. We used this method to isolate hepatocytes and kidney proximal epithelial cells-specific transcriptional response to a dietary challenge without interfering with the tissue architecture, and to identify multiple hepatocyte-secreted ccfRNAs in plasma. We also identified transfer of these hepatic derived ccfRNAs to adipose tissue, where they likely serve as a buffering mechanism to maintain cholesterol and lipid homeostasis. Our findings directly demonstrate in-vivo transfer of RNAs between tissues and highlight its implications for endocrine signaling and homeostasis.