RT Journal Article
SR Electronic
T1 Multiplex Chromatin Interaction Analysis with Single-Molecule Precision
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 252049
DO 10.1101/252049
A1 Meizhen Zheng
A1 Simon Zhongyuan Tian
A1 Rahul Maurya
A1 Byoungkoo Lee
A1 Minji Kim
A1 Daniel Capurso
A1 Emaly Piecuch
A1 Liang Gong
A1 Jacqueline Jufen Zhu
A1 Chee Hong Wong
A1 Chew Yee Ngan
A1 Ping Wang
A1 Xiaoan Ruan
A1 Chia-Lin Wei
A1 Yijun Ruan
YR 2018
UL http://biorxiv.org/content/early/2018/01/25/252049.abstract
AB We describe a microfluidics-based strategy for genome-wide analysis of multiplex chromatin interactions with single-molecule precision. In multiplex chromatin interaction analysis (multi-ChIA), individual chromatin complexes are partitioned into droplets that contain a gel bead with unique DNA barcode, in which tethered chromatin DNA fragments are barcoded and amplified for sequencing and mapping to demarcate chromatin contacts. Thus, multi-ChIA has the unprecedented ability to uncover multiplex chromatin interactions at single-molecule level, which has been impossible using previous methods that rely on analyzing pairwise contacts via proximity ligation. We demonstrate that multiplex chromatin interactions predominantly contribute to topologically associated domains, and clusters of gene promoters and enhancers provide a fundamental topological framework for co-transcriptional regulation.