RT Journal Article
SR Electronic
T1 <em>Plasmodium</em> condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 674028
DO 10.1101/674028
A1 Rajan Pandey
A1 Steven Abel
A1 Matthew Boucher
A1 Richard J Wall
A1 Mohammad Zeeshan
A1 Edward Rea
A1 Aline Freville
A1 Xueqing Maggie Lu
A1 Declan Brady
A1 Emilie Daniel
A1 Rebecca R. Stanway
A1 Sally Wheatley
A1 Gayani Batugedara
A1 Thomas Hollin
A1 Andrew R. Bottrill
A1 Dinesh Gupta
A1 Anthony A. Holder
A1 Karine G. Le Roch
A1 Rita Tewari
YR 2019
UL http://biorxiv.org/content/early/2019/06/17/674028.abstract
AB Condensin is a multi-subunit protein complex that regulates chromosome organization, segregation and condensation during cell division in eukaryotes. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin during endomitosis in schizogony and endoreduplication in male gametogenesis. SMC2 and SMC4 localize at discrete foci during schizogony, and with a diffuse nuclear distribution during male gametogenesis. ChIP-seq analyses suggest a centromeric location of SMC2/SMC4 only during schizogony. Co-immunoprecipitation data reveal the presence of both condensin complex I and II during male gametogenesis, but only the SMC2/SMC4 heterodimer during schizogony. Finally, knockdown of smc2 and smc4 gene expression revealed their essential roles in parasite proliferation and transmission. This study shows that condensin core subunits (SMC2/SMC4) have differential complex and distinct functions at different stages of the parasite life cycle.