PT  - JOURNAL ARTICLE
AU  - Max Koppers
AU  - Roberta Cagnetta
AU  - Toshiaki Shigeoka
AU  - Lucia C.S. Wunderlich
AU  - Sixian Zhao
AU  - Michael S. Minett
AU  - Anaïs Bellon
AU  - Clemens F. Kaminski
AU  - John. G. Flanagan
AU  - Christine E. Holt
TI  - Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
AID  - 10.1101/673798
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 673798
4099  - http://biorxiv.org/content/early/2019/06/17/673798.short
4100  - http://biorxiv.org/content/early/2019/06/17/673798.full
AB  - During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors bind to distinct sets of mRNAs and RNA-binding proteins. Cue stimulation induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs in retinal axon growth cones. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by simultaneous exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and provide a general model for the rapid, localized and selective control of cue-induced translation.