@article {Stewart672055, author = {Luke T. Stewart and Kavitha Abiraman and John C. Chatham and Lori L. McMahon}, title = {Increased O-GlcNAcylation rapidly decreases GABAAR currents in hippocampus yet depresses neuronal output}, elocation-id = {672055}, year = {2019}, doi = {10.1101/672055}, publisher = {Cold Spring Harbor Laboratory}, abstract = {O-GlcNAcylation, a post-translational modification involving O-linkage of β-N-acetylglucosamine to Ser/Thr residues on target proteins, is increasingly recognized as a critical regulator of brain function in health and disease. Enzymes that catalyze O-GlcNAcylation are found at both presynaptic and postsynaptic sites, and O-GlcNAcylated proteins localize to synaptosomes. Acute increase in O-GlcNAcylation induces long-term depression (LTD) of excitatory transmission at hippocampal CA3-CA1 synapses, and depresses hyperexcitable circuits in vitro and in vivo. Yet, no study has investigated how O-GlcNAcylation modulates the efficacy of inhibitory neurotransmission. Here we show an acute increase in O-GlcNAc dampens GABAergic currents onto principal cells in rodent hippocampus likely through a postsynaptic mechanism, and increases the excitation/inhibition balance. However, the overall effect of increased O-GlcNAc is reduced synaptically-driven spike probability via decreased intrinsic excitability and synaptic depression. Our results position O-GlcNAcylation as a novel regulator of the excitation/inhibition balance and neuronal output.}, URL = {https://www.biorxiv.org/content/early/2019/06/17/672055}, eprint = {https://www.biorxiv.org/content/early/2019/06/17/672055.full.pdf}, journal = {bioRxiv} }