PT - JOURNAL ARTICLE AU - Toro-Nahuelpan, Mauricio AU - Zagoriy, Ievgeniia AU - Senger, Fabrice AU - Blanchoin, Laurent AU - Théry, Manuel AU - Mahamid, Julia TI - Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies AID - 10.1101/676189 DP - 2019 Jan 01 TA - bioRxiv PG - 676189 4099 - http://biorxiv.org/content/early/2019/06/19/676189.short 4100 - http://biorxiv.org/content/early/2019/06/19/676189.full AB - Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex patterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell 3D-structural characterization of the underlying molecular machinery.