PT  - JOURNAL ARTICLE
AU  - Toro-Nahuelpan, Mauricio
AU  - Zagoriy, Ievgeniia
AU  - Senger, Fabrice
AU  - Blanchoin, Laurent
AU  - Théry, Manuel
AU  - Mahamid, Julia
TI  - Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
AID  - 10.1101/676189
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 676189
4099  - http://biorxiv.org/content/early/2019/06/19/676189.short
4100  - http://biorxiv.org/content/early/2019/06/19/676189.full
AB  - Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex patterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell 3D-structural characterization of the underlying molecular machinery.