RT Journal Article
SR Electronic
T1 Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 676189
DO 10.1101/676189
A1 Toro-Nahuelpan, Mauricio
A1 Zagoriy, Ievgeniia
A1 Senger, Fabrice
A1 Blanchoin, Laurent
A1 Théry, Manuel
A1 Mahamid, Julia
YR 2019
UL http://biorxiv.org/content/early/2019/06/19/676189.abstract
AB Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex patterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell 3D-structural characterization of the underlying molecular machinery.