PT  - JOURNAL ARTICLE
AU  - Nicholas C Lammers
AU  - Vahe Galstyan
AU  - Armando Reimer
AU  - Sean A Medin
AU  - Chris H Wiggins
AU  - Hernan G Garcia
TI  - Multimodal transcriptional control of pattern formation in embryonic development
AID  - 10.1101/335919
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 335919
4099  - http://biorxiv.org/content/early/2019/06/20/335919.short
4100  - http://biorxiv.org/content/early/2019/06/20/335919.full
AB  - Predicting developmental outcomes from regulatory DNA sequence and transcription factor patterns remains an open challenge in physical biology. Using stripe 2 of the even-skipped gene in Drosophila embryos as a case study, we dissect the regulatory forces underpinning a key step along the developmental decision-making cascade: the generation of cytoplasmic mRNA patterns via the control of transcription in individual cells. Using live imaging and computational approaches, we found that the transcriptional burst frequency is modulated across the stripe to control the mRNA production rate. However, we discovered that bursting alone cannot quantitatively recapitulate the formation of the stripe, and that control of the window of time over which each nucleus transcribes even-skipped plays a critical role in stripe formation. Theoretical modeling revealed that these regulatory strategies—bursting and the time window—obey different kinds of regulatory logic, suggesting that the stripe is shaped by the interplay of two distinct underlying molecular processes.