PT - JOURNAL ARTICLE AU - Helen Farrants AU - Miroslaw Tarnawski AU - Thorsten G. Müller AU - Shotaro Otsuka AU - Julien Hiblot AU - Birgit Koch AU - Moritz Kueblbeck AU - Hans-Georg Kräusslich AU - Jan Ellenberg AU - Kai Johnsson TI - Chemogenetic Control of Nanobodies AID - 10.1101/683557 DP - 2019 Jan 01 TA - bioRxiv PG - 683557 4099 - http://biorxiv.org/content/early/2019/06/27/683557.short 4100 - http://biorxiv.org/content/early/2019/06/27/683557.full AB - We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.