PT  - JOURNAL ARTICLE
AU  - Uday Madaan
AU  - Lionel Faure
AU  - Albar Chowdhury
AU  - Shahrear Ahmed
AU  - Emma J. Ciccarelli
AU  - Tina L. Gumienny
AU  - Cathy Savage-Dunn
TI  - Feedback regulation of BMP signaling by <em>C. elegans</em> cuticle collagens
AID  - 10.1101/686592
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 686592
4099  - http://biorxiv.org/content/early/2019/06/28/686592.short
4100  - http://biorxiv.org/content/early/2019/06/28/686592.full
AB  - Cellular responsiveness to environmental cues, including changes in extracellular matrix (ECM), is critical for normal processes such as development and wound healing, but can go awry, as in oncogenesis and fibrosis. One type of molecular pathway allowing this responsiveness is the bone morphogenetic protein (BMP) signaling pathway. Due to their broad and potent functions, BMPs and their signaling pathways are highly regulated at multiple levels. In Caenorhabditis elegans, the BMP ligand DBL-1 is a major regulator of body size. We have previously shown that DBL-1/BMP signaling determines body size through transcriptional regulation of cuticle collagen genes. We have now obtained evidence of feedback regulation of DBL-1/BMP by collagen genes. We analyzed four DBL-1-regulated collagen genes that affect body size. Here we show that inactivation of any one of these cuticle collagen genes reduces DBL-1/BMP signaling, as measured by a Smad activity reporter. Furthermore, we find that depletion of these collagens reduces GFP::DBL-1 fluorescence, and acts unexpectedly at the level of dbl-1 transcription. We therefore conclude that cuticle, a type of ECM, impinges on DBL-1/BMP expression and signaling. In contrast to other characterized examples, however, the feedback regulation of DBL-1/BMP signaling by collagens is likely to be contact-independent, due to the physical separation of the cuticle from DBL-1-expressing cells in the ventral nerve cord. Our results provide an entry point into a novel mechanism of regulation of BMP signaling, with broader implications for mechanical regulation of gene expression in general.