TY - JOUR T1 - Increased autophagy in ephrinB2 deficient osteocytes is associated with hypermineralized, brittle bones JF - bioRxiv DO - 10.1101/260711 SP - 260711 AU - Christina Vrahnas AU - Toby A Dite AU - Niloufar Ansari AU - Blessing Crimeen-Irwin AU - Huynh Nguyen AU - Mark R Forwood AU - Yifang Hu AU - Mika Ikegame AU - Keith R Bambery AU - Cyril Petibois AU - Mark J Tobin AU - Gordon K Smyth AU - Jonathan S Oakhill AU - T John Martin AU - Natalie A Sims Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/02/05/260711.abstract N2 - Mineralized bone forms when collagen-containing osteoid accrues hydroxyapatite crystals. This process has two phases: a rapid initiation (primary mineralization), followed by slower accrual of mineral (secondary mineralization) that continues until that portion of bone is renewed by remodelling. Within the bone matrix is an interconnected network of cells termed osteocytes. These cells are derived from bone-forming osteoblasts. This cellular transition requires expression of ephrinB2, and we were intrigued about why ephrinB2 continues to be expressed at high levels in mature osteocytes. To determine its function in osteocytes, we developed an osteocyte-specific ephrinB2 null mouse and found they exhibited a brittle bone phenotype. This was not caused by a change in bone mass, but by an intrinsic defect in the strength of the bone material. Although the initiation of osteoid mineralization occurred at a normal rate, the process of secondary mineralization was accelerated in these mice. The maturing mineralized bone matrix incorporated mineral and carbonate more rapidly than controls, indicating that osteocytic ephrinB2 suppresses mineral accumulation in bone. No known regulators of mineralization were modified in the bone of these mice. However, RNA sequencing showed differential expression of a group of autophagy-associated genes, and increased autophagic flux was confirmed in ephrinB2 knockdown osteocytes. This indicates that the process of secondary mineralization in bone makes use of autophagic machinery in a manner that is limited by ephrinB2 in osteocytes, and that this process may be disrupted in conditions of bone fragility. ER -