PT  - JOURNAL ARTICLE
AU  - Federico Baldini
AU  - Johannes Hertel
AU  - Estelle Sandt
AU  - Cyrille C. Thinnes
AU  - Lorieza Neuberger-Castillo
AU  - Lukas Pavelka
AU  - Fay Betsou
AU  - Rejko Krüger
AU  - Ines Thiele
AU  - on behalf of the NCER-PD Consortium
TI  - Parkinson’s disease-associated alterations of the gut microbiome can invoke disease-relevant metabolic changes
AID  - 10.1101/691030
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 691030
4099  - http://biorxiv.org/content/early/2019/07/02/691030.short
4100  - http://biorxiv.org/content/early/2019/07/02/691030.full
AB  - Parkinson’s disease (PD) is a systemic disease clinically defined by the degeneration of dopaminergic neurons in the brain. While alterations in the gut microbiome composition have been reported in PD, their functional consequences remain unclear. Herein, we first analysed the gut microbiome of patients and healthy controls by 16S rRNA gene sequencing of stool samples from the Luxembourg Parkinson’s study (n=147 typical PD cases, n=162 controls). All individuals underwent detailed clinical assessment, including neurological examinations and neuropsychological tests followed by self-reporting questionnaires. Second, we predicted the potential secretion for 129 microbial metabolites through personalised metabolic modelling using the microbiome data and genome-scale metabolic reconstructions of human gut microbes. Our key results include: 1. eight genera and nine species changed significantly in their relative abundances between PD patients and healthy controls. 2. PD-associated microbial patterns statistically depended on sex, age, BMI, and constipation. The relative abundances of Bilophila and Paraprevotella were significantly associated with the Hoehn and Yahr staging after controlling for the disease duration. In contrast, dopaminergic medication had no detectable effect on the PD microbiome composition. 3. Personalised metabolic modelling of the gut microbiomes revealed PD-associated metabolic patterns in secretion potential of nine microbial metabolites in PD, including increased methionine and cysteinylglycine. The microbial pantothenic acid production potential was linked to the presence of specific non-motor symptoms and attributed to individual bacteria, such as Akkermansia muciniphila and Bilophila wardswarthia. Our results suggest that PD-associated alterations of gut microbiome could translate into functional differences affecting host metabolism and disease phenotype.