RT Journal Article
SR Electronic
T1 Label-retention expansion microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 687954
DO 10.1101/687954
A1 Xiaoyu Shi
A1 Qi Li
A1 Zhipeng Dai
A1 Arthur A. Tran
A1 Siyu Feng
A1 Alejandro D. Ramirez
A1 Zixi Lin
A1 Xiaomeng Wang
A1 Tracy T. Chow
A1 Ian B. Seiple
A1 Bo Huang
YR 2019
UL http://biorxiv.org/content/early/2019/07/02/687954.abstract
AB Expansion microscopy (ExM) improves the resolution of fluorescence microscopy by physically expanding the sample embedded in a hydrogel1–4. Since its invention, ExM has been successfully applied to a wide range of cell, tissue and animal samples 2–9. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency protein labeling using enzymatic tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with super-resolution Stochastic Optical Reconstruction Microscopy (STORM), we have achieved 5 nm resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.