PT  - JOURNAL ARTICLE
AU  - Xiaoran Xin
AU  - Lei Lei
AU  - Yunzhen Zheng
AU  - Tian Zhang
AU  - Sai Venkatesh Pingali
AU  - Hugh O’Neill
AU  - Daniel J. Cosgrove
AU  - Shundai Li
AU  - Ying Gu
TI  - CELLULOSE SYNTHASE INTERACTIVE1- and Microtubule-Dependent Cell Wall Architecture Is Required for Acid Growth in <em>Arabidopsis Hypocotyls</em>
AID  - 10.1101/692202
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 692202
4099  - http://biorxiv.org/content/early/2019/07/03/692202.1.short
4100  - http://biorxiv.org/content/early/2019/07/03/692202.1.full
AB  - Auxin-induced cell elongation relies in part on the acidification of the cell wall, a process known as acid growth that presumably triggers expansin-mediated wall loosening via altered interactions between cellulose microfibrils. Cellulose microfibrils are a major determinant for anisotropic growth and they provide the scaffold for cell wall assembly. Little is known about how acid growth depends on cell wall architecture. To explore the relationship between acid growth-mediated cell elongation and plant cell wall architecture, two mutants (jia1-1 and csi1-3) that are defective in cellulose biosynthesis and cellulose microfibril organization were analyzed. The study revealed that cell elongation is dependent on CSI1-mediated cell wall architecture but not on the overall crystalline cellulose content. We observed a correlation between loss of crossed-polylamellate walls and loss of auxin- and fusicoccin-induced cell growth in csi1-3. Furthermore, induced loss of crossed-polylamellate walls via disruption of cortical microtubule mimics the effect of csi1 in acid growth. We hypothesize that CSI1- and microtubule-dependent crossed-polylamellate walls are required for acid growth in Arabidopsis hypocotyls.