RT Journal Article
SR Electronic
T1 Generating genomic platforms to study <em>Candida albicans</em> pathogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 261628
DO 10.1101/261628
A1 Mélanie Legrand
A1 Sophie Bachellier-Bassi
A1 Keunsook K. Lee
A1 Yogesh Chaudhari
A1 Hélène Tournu
A1 Laurence Arbogast
A1 Hélène Boyer
A1 Murielle Chauvel
A1 Vitor Cabral
A1 Corinne Maufrais
A1 Audrey Nesseir
A1 Irena Maslanka
A1 Emmanuelle Permal
A1 Tristan Rossignol
A1 Louise A. Walker
A1 Ute Zeidler
A1 Sadri Znaidi
A1 Floris Schoeters
A1 Charlotte Majgier
A1 Renaud A. Julien
A1 Laurence Ma
A1 Magali Tichit
A1 Christiane Bouchier
A1 Patrick Van Dijck
A1 Carol A. Munro
A1 Christophe d’Enfert
YR 2018
UL http://biorxiv.org/content/early/2018/02/08/261628.abstract
AB The advent of the genomic era has made elucidating gene function at large scale a pressing challenge. ORFeome collections, whereby almost all ORFs of a given species are cloned and can be subsequently leveraged in multiple functional genomic approaches, represent valuable resources towards this endeavor. Here we provide novel, genome-scale tools for the study of Candida albicans, a commensal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5,102 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at http://candidaorfeome.eu. We also engineered 49 expression vectors with a choice of promoters, tags, and selection markers and demonstrated their applicability to the study of target ORFs transferred from the C. albicans ORFeome. In addition, the use of the ORFeome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were engineered, validated and used in a proof of concept experiment. These unique and valuable resources should greatly facilitate future functional studies in C. albicans and the elucidation of mechanisms that underlie its pathogenicity.