TY - JOUR T1 - Mutations in a single signaling pathway allow growth on a different solvent than water JF - bioRxiv DO - 10.1101/692889 SP - 692889 AU - Caroline Kampmeyer AU - Jens V. Johansen AU - Christian Holmberg AU - Magnus Karlson AU - Sarah K. Gersing AU - Heloisa N. Bordallo AU - Birthe B. Kragelund AU - Mathilde H. Lerche AU - Isabelle Jourdain AU - Jakob R. Winther AU - Rasmus Hartmann-Petersen Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/07/04/692889.abstract N2 - Since life is completely dependent on water, it is difficult to gauge the impact of solvent change. To analyze the role of water as a solvent in biology, we replaced water with heavy water (D2O), and investigated the biological effects by a wide range of techniques, using the fission yeast Schizosaccharomyces pombe as model organism. We show that high concentrations of D2O lead to altered glucose metabolism, growth retardation, and inhibition of meiosis. However, mitosis and overall cell viability were only slightly affected. After prolonged incubation in D2O, cells displayed gross morphological changes, thickened cell walls as well as aberrant septa and cytoskeletal organization. RNA sequencing revealed that D2O causes a strong downregulation of most tRNAs and triggers activation of the general stress response pathway. Genetic screens identified several D2O sensitive mutants, while mutants compromised in the cell integrity pathway, including the protein kinase genes pmk1, mkh1, pek1 and pck2, that control cell wall biogenesis, were more tolerant to D2O. We speculate that D2O affects the phospholipid membrane or cell wall glycans causing an activation of the cell integrity pathway. In conclusion, the effects of solvent replacement are pleiotropic but the D2O-triggered activation of the cell integrity pathway and subsequent increased deposition of cell wall material and septation problems appear most critical for the cell growth defects. ER -