PT  - JOURNAL ARTICLE
AU  - Brian S. Hercyk
AU  - Julie T. Rich-Robinson
AU  - Ahmad S. Mitoubsi
AU  - Marcus A. Harrell
AU  - Maitreyi E. Das
TI  - A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis
AID  - 10.1101/364786
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 364786
4099  - http://biorxiv.org/content/early/2019/07/05/364786.short
4100  - http://biorxiv.org/content/early/2019/07/05/364786.full
AB  - Cdc42, a conserved regulator of cell polarity, is activated by two GEFs, Gef1 and Scd1, in fission yeast. While gef1 and scd1 mutants exhibit distinct phenotypes, how they do so is unclear given that they activate the same GTPase. Using the GEF localization pattern during cytokinesis as a paradigm, we report a novel interplay between Gef1 and Scd1 that spatially modulates Cdc42. We find that Gef1 promotes Scd1 localization to the division site during cytokinesis and to the new end during polarized growth through the recruitment of the scaffold Scd2 via a Cdc42 feedforward pathway. Gef1-mediated Scd1 recruitment at the new end enables the transition from monopolar to bipolar growth. Reciprocally, Scd1 restricts Gef1 localization to prevent ectopic Cdc42 activation during cytokinesis to promote cell separation and during interphase to maintain cell shape. Our findings reveal an elegant regulatory pattern in which Gef1 establishes new sites of Scd1-mediated Cdc42 activity, while Scd1 restricts Gef1 to functional sites. We propose that crosstalk between GEFs is a conserved mechanism that orchestrates Cdc42 activation during complex cellular processes.Summary Statement Cdc42 GEFs Gef1 and Scd1 crosstalk to fine-tune Cdc42 activity. This crosstalk promotes bipolar growth and maintains cell shape in fission yeast.