RT Journal Article
SR Electronic
T1 Magnetic resonance measurements of sub-cellular membrane structures in live and fixed neural tissue
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 694661
DO 10.1101/694661
A1 Nathan H. Williamson
A1 Rea Ravin
A1 Dan Benjamini
A1 Hellmut Merkle
A1 Melanie Falgairolle
A1 Michael J. O’Donovan
A1 Dvir Blivis
A1 Dave Ide
A1 Teddy X. Cai
A1 Nima S. Ghorashi
A1 Ruiliang Bai
A1 Peter J. Basser
YR 2019
UL http://biorxiv.org/content/early/2019/07/07/694661.abstract
AB We develop magnetic resonance (MR) methods for measuring real-time changes of tissue microstructure and membrane permeability of live and fixed neural tissue. Diffusion and exchange MR measurements are performed using the large static gradient produced by a single-sided permanent magnet. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted within membrane structures in the range of 200–1400 nm at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between sub-cellular structures and free environments at 100 s−1.