PT - JOURNAL ARTICLE AU - Rakesh Pathak AU - Priyanka Singh AU - Sudha Ananthakrishnan AU - Sarah Adamczyk AU - Olivia Schimmel AU - Chhabi K. Govind TI - <em>Saccharomyces cerevisiae</em> histone Chaperones FACT and Spt6 modulate Pol II and histone occupancy genome-wide AID - 10.1101/265074 DP - 2018 Jan 01 TA - bioRxiv PG - 265074 4099 - http://biorxiv.org/content/early/2018/02/13/265074.short 4100 - http://biorxiv.org/content/early/2018/02/13/265074.full AB - Histone chaperones, chromatin remodelers, and histone modifying complexes play a critical role in alleviating the nucleosomal barrier. Here, we have examined the role of two highly conserved yeast (Saccharomyces cerevisiae) histone chaperones, FACT and Spt6, in regulating transcription and histone occupancy. We show that the H3 tail contributes to the recruitment of FACT to coding sequences in a manner dependent on acetylation. We found that deleting a H3 HAT Gcn5 or mutating lysines on the H3 tail impairs FACT recruitment at ADH1 and ARG1 genes. However, deleting the H4 tail or mutating the H4 lysines failed to dampen FACT occupancy in coding regions. Additionally, we show that FACT-depletion greatly reduces Pol II occupancy in the 5’ ends genome-wide. By contrast, Spt6-depletion led to reduction in Pol II occupancy towards the 3’ end, in a manner dependent on the gene-length. Severe transcription and histone eviction defects were also observed in a strain that was impaired for Spt6 recruitment (spt6Δ202) and depleted of FACT. Importantly, the severity of the defect strongly correlated with WT Pol II occupancies at these genes, indicating critical roles of Spt6 and Spt16 in promoting high-level transcription. Collectively, our study shows cooperation, as well as redundancy between chaperones, FACT and Spt6, in regulating transcription and chromatin in coding regions of transcribed genes.