@article {Vincent677260, author = {Ben J. Vincent and Gavin R. Rice and Gabriella M. Wong and William J. Glassford and Kayla I. Downs and Jessica L. Shastay and Kenechukwu Charles-Obi and Malini Natarajan and Madelaine Gogol and Julia Zeitlinger and Mark Rebeiz}, title = {An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia}, elocation-id = {677260}, year = {2019}, doi = {10.1101/677260}, publisher = {Cold Spring Harbor Laboratory}, abstract = {During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially in tissues that undergo rapid evolutionary change. The terminalia (genital and anal structures) of Drosophila melanogaster and its close relatives exhibit dramatic changes in morphology between species. While previous studies have found network components important for patterning the larval genital disc, the networks governing adult structures during pupal development have remained uncharted. Here, we performed RNA-seq in whole Drosophila melanogaster terminalia followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the terminalia is highly patterned during pupal stages and that specific transcription factors mark separate structures and substructures. Our results are housed online in a searchable database (flyterminalia.pitt.edu) where they can serve as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia.Summary We performed RNA-seq in whole Drosophila melanogaster terminalia (genitalia and analia) followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the pupal terminalia is highly patterned with specific transcription factors marking separate structures and substructures. Our results are housed online in a searchable database (flyterminalia.pitt.edu) where they can serve as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia.}, URL = {https://www.biorxiv.org/content/early/2019/07/10/677260}, eprint = {https://www.biorxiv.org/content/early/2019/07/10/677260.full.pdf}, journal = {bioRxiv} }